Hi Conor, We have a lot shorter notice time for the perfusion, we might be just notified in the morning. I think 2-3 hours’ notice to the engineers is more accurate. Thanks, Agnes From: Conor Parks &lt;cp20(a)sanger.ac.uk&gt; Sent: 10 January 2024 16:41 To: Madelyn Moy &lt;mm59(a)sanger.ac.uk&gt;uk>; April Rose Foster &lt;af23(a)sanger.ac.uk&gt;uk>; &#39;cellgenrsflab(a)sanger.ac.uk&#39; &lt;cellgenrsflab(a)sanger.ac.uk&gt; Cc: Laure Gambardella &lt;lg24(a)sanger.ac.uk&gt;uk>; Muzlifah Haniffa &lt;mh32(a)sanger.ac.uk&gt; Subject: [Cellgenrsflab] Re: Upcoming electrical works, check dates. Hi all, @Madelyn I’ve asked them to do the Tuesday/Wednesday/Thursday instead. @April I’ve indicated the incubators and rotor cannot be turned off at all and that to if possible do weekend work or delay for a week or two will see what dates they come back with. @Elena, I’ve indicated that if a perfusion experiment occurs we’ll have to cancel the testing but that we usually only have 24 hours’ notice of this. BW, Conor From: Madelyn Moy <mm59@sanger.ac.uk<mailto:mm59@sanger.ac.uk>> Sent: 09 January 2024 15:33 To: April Rose Foster <af23@sanger.ac.uk<mailto:af23@sanger.ac.uk>>; Conor Parks <cp20@sanger.ac.uk<mailto:cp20@sanger.ac.uk>>; &#39;cellgenrsflab(a)sanger.ac.uk&#39; <cellgenrsflab@sanger.ac.uk<mailto:cellgenrsflab@sanger.ac.uk>> Cc: cellgenlab@sanger.ac.uk<mailto:cellgenlab@sanger.ac.uk>; Laure Gambardella <lg24@sanger.ac.uk<mailto:lg24@sanger.ac.uk>>; Muzlifah Haniffa <mh32@sanger.ac.uk<mailto:mh32@sanger.ac.uk>> Subject: Re: [Cellgenrsflab] Re: Upcoming electrical works, check dates. Hi Conor, Jack and I will have important cultures in the RSF tissue processing room incubators during this time. We think it should be fine for the works to occur if the incubators will only be off for 15 minutes, but could we please have contingencies in place (i.e., backup incubators) in case there are any issues getting the incubators back on/up to temperature? Perhaps we do the tissue processing and organoid rooms on different days so the other room’s incubators can be available if need be? It would be extremely detrimental to both our projects if we lost these cultures. Additionally, could we ask for the works in the tissue processing room to be on a Tuesday, Wednesday, or Thursday?. They are currently scheduled for a Monday (5th), but Mondays and Fridays tend to be our lab intensive days. Thank you. Best, Maddy From: April Rose Foster <af23@sanger.ac.uk<mailto:af23@sanger.ac.uk>> Date: Tuesday, January 9, 2024 at 3:13 PM To: Conor Parks <cp20@sanger.ac.uk<mailto:cp20@sanger.ac.uk>>, &quot;&#39;cellgenrsflab(a)sanger.ac.uk&#39;&quot; <cellgenrsflab@sanger.ac.uk<mailto:cellgenrsflab@sanger.ac.uk>> Cc: "cellgenlab@sanger.ac.uk<mailto:cellgenlab@sanger.ac.uk>" <cellgenlab@sanger.ac.uk<mailto:cellgenlab@sanger.ac.uk>>, Laure Gambardella <lg24@sanger.ac.uk<mailto:lg24@sanger.ac.uk>>, Muzlifah Haniffa <mh32@sanger.ac.uk<mailto:mh32@sanger.ac.uk>> Subject: [Cellgenrsflab] Re: Upcoming electrical works, check dates. Hi Conor, This would be an issue for our team working in the RSF organoid room. We are going to be in the middle of a very important organoid differentiation as part of paper revision experiments. These experiments will be reliant on the incubators and rotator working throughout. Sorry to be a nuisance in the planning of this works but maybe there can be some rearrangement until we have passed this critical differentiation period. Best wishes, April April Rose Foster, PhD Staff Scientist Haniffa Lab, T298 Cellular Genetics Wellcome Sanger Institute Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK From: Elena Prigmore <ep2@sanger.ac.uk<mailto:ep2@sanger.ac.uk>> Date: Tuesday, 9 January 2024 at 11:08 To: Conor Parks <cp20@sanger.ac.uk<mailto:cp20@sanger.ac.uk>>, &quot;&#39;cellgenrsflab(a)sanger.ac.uk&#39;&quot; <cellgenrsflab@sanger.ac.uk<mailto:cellgenrsflab@sanger.ac.uk>> Cc: "cellgenlab@sanger.ac.uk<mailto:cellgenlab@sanger.ac.uk>" <cellgenlab@sanger.ac.uk<mailto:cellgenlab@sanger.ac.uk>> Subject: [Cellgenrsflab] Re: Upcoming electrical works, check dates. Hi Conor, The only thing would be if there was a perfusion going on we would not be able to turn the power off, even for 15 mins. We can’t really predict when this might happen as it depends on the availability of kidneys. I see there is a range of dates so hopefully there can be some flexibility to accommodate a perfusion experiment. Also we wouldn’t want to decline kidney samples just if this testing work had been planned. Perhaps we could add an exception for the perfusion room. Thanks for your help with this, Best wishes, Elena From: Conor Parks <cp20@sanger.ac.uk<mailto:cp20@sanger.ac.uk>> Sent: 09 January 2024 10:17 To: &#39;cellgenrsflab(a)sanger.ac.uk&#39; <cellgenrsflab@sanger.ac.uk<mailto:cellgenrsflab@sanger.ac.uk>> Cc: cellgenlab@sanger.ac.uk<mailto:cellgenlab@sanger.ac.uk> Subject: Upcoming electrical works, check dates. Hi all, There is upcoming wire testing in the RSF which must be carried out as a legal requirement, there is some wiggle room on the dates. Each electrical circuit in the room will be turned off for up to 15 minutes. Can everyone check the dates below and let me know: 1. If there is any critical work taking place on that day that cannot be interrupted. 2. If there’s any equipment in use that wouldn’t be able to be turned off. (Incubators should be fine as they won’t be opening them while the power is off). Room No Time taken Date R1-42 3 Hours 01/02/2024 R1-43 3 Hours 01/02/2024 R1-45 3 Hours 01/02/2024 R1-46 & R1-46A 3 Hours 02/02/2024 R-147 3 Hours 02/02/2024 R1-41, R1-44 3 Hours 05/02/2024 R1-26, R1-25 3 Hours 05/02/2024 R1-54 3 Hours 06/02/2024 R1-53 3 Hours 06/02/2024 R1-52 3 Hours 07/02/2024 R1-51 3 Hours 07/02/2024 R1-50 3 Hours 07/02/2024 Air Handling Units 3 hours 08/02/2024 BW, Conor -- Conor Parks Scientific Manager | Wellcome Sanger Institute Wet Lab Support Team (T279) Behjati Team (T274)