Hi Martin,
Am I correct in understanding that if Aaron and Max generate the hosted
cellxgene link then all that you need is that link along with the basic lab
info? Basically, it would save the time of submitting the object and other
details, right? Asking only to try and streamline work for Aaron and you as
well!
Best,
Jonah
On Wed, Jun 2, 2021 at 2:21 AM Martin Prete <mp33(a)sanger.ac.uk> wrote:
Hi Aaron,
Vladimir is on annual leave this week. We can add Blish Lab’s new dataset
without the cellxgene link and we’ll update that later.
I’ll need a title, an object and a source link (pre-print or published
article). That’s pretty much it. I think:
- the object is
https://urldefense.proofpoint.com/v2/url?u=https-3A__drive.google.com_file_…
[
drive.google.com]
<https://urldefense.proofpoint.com/v2/url?u=https-3A__drive.google.com_file_d_1cG60rcbff-2Dh-5F3ZoAM2GkJTttIQR4RWb9_view-3Fusp-3Dsharing&d=DwMFaQ&c=D7ByGjS34AllFgecYw0iC6Zq7qlm8uclZFI0SqQnqBo&r=9KyXPtoyUP8edjdvA3wxPg&m=K5ZPsdQyE0rEJ3G0OQbGKwCp5td6zRJhHXhWF3KrFqM&s=-fxLTy7hu5EHV8QxohQfoewblWTzSxF7SAYZXHSyuug&e=>
- source would be
https://urldefense.proofpoint.com/v2/url?u=https-3A__doi.org_10.1084_jem.20…
Main thing missing is a title. We already have another object for Blish
Lab (Peripheral Blood Mononuclear Cells) so I guess the link to the team
will remain the same.
Please, confirm that and I’ll upload the object (RDS) and add the entry
for this new dataset.
Kind regards,
Martin.-
*From: *Aaron James Wilk <awilk(a)stanford.edu>
*Date: *Tuesday, 1 June 2021 at 18:34
*To: *Vladimir Kiselev <vk6(a)sanger.ac.uk>uk>, Jonah Cool <
jcool(a)chanzuckerberg.com>
*Cc: *"cv19caingest(a)sanger.ac.uk" <cv19caingest(a)sanger.ac.uk>uk>, Martin
Prete <mp33(a)sanger.ac.uk>uk>, Maximilian Lombardo <
mlombardo(a)chanzuckerberg.com>gt;, Kirtana Veeraraghavan <
kveeraraghavan(a)chanzuckerberg.com>
*Subject: *Re: [Cv19caingest] Blish lab: New single-cell dataset for
COVID-19 cell atlas [EXT]
Hi Vlad,
I just met with Max from Biohub and will start to work on getting this
dataset curated for cellxgene. In the meantime, is there anything you need
from me to get the object hosted on the cell atlas, or will you wait for
the cellxgene link to post it?
Thanks,
Aaron
*From: *Vladimir Kiselev <vk6(a)sanger.ac.uk>
*Date: *Wednesday, May 26, 2021 at 7:28 AM
*To: *Jonah Cool <jcool(a)chanzuckerberg.com>om>, Aaron James Wilk <
awilk(a)stanford.edu>
*Cc: *"cv19caingest(a)sanger.ac.uk" <cv19caingest(a)sanger.ac.uk>uk>, Martin
Prete <mp33(a)sanger.ac.uk>uk>, Maximilian Lombardo <
mlombardo(a)chanzuckerberg.com>gt;, Kirtana Veeraraghavan <
kveeraraghavan(a)chanzuckerberg.com>
*Subject: *Re: [Cv19caingest] Blish lab: New single-cell dataset for
COVID-19 cell atlas [EXT]
Hi All,
Please let us know when the links to visualisations are ready and share
them with us. We will then add them to the covid19atlas portal.
Many thanks,
Cheers,
Vlad
--
*Vlad Kiselev*
CellGen Informatics
<http://www.sanger.ac.uk/science/groups/cellular-genetics-informatics>
Team Leader
Wellcome Sanger Institute
Hinxton, Cambridge, CB10 1SA
Phone: +44(0)1223496850
Message: Mattermost <https://mattermost.sanger.ac.uk/cellgeninf> or Slack
[
cellgeni.slack.com]
<https://urldefense.proofpoint.com/v2/url?u=https-3A__cellgeni.slack.com_&d=DwMGaQ&c=D7ByGjS34AllFgecYw0iC6Zq7qlm8uclZFI0SqQnqBo&r=xVM0Oj0byXJqPMlhvbopPQeNDkgpFF7SJ9Qqo_aizig&m=lKsEU9Bd6rtqTfZCkUGZS3DXC1qAc6ZkHRqIuLdnYoY&s=quvLvlGreFDL8J9uzAcrDb6epi9urz9C-YHJx0_vb2Y&e=>
Web:
https://urldefense.proofpoint.com/v2/url?u=https-3A__wikiselev.github.io&am…
[wikiselev.github.io]
<https://urldefense.proofpoint.com/v2/url?u=https-3A__wikiselev.github.io&d=DwMGaQ&c=D7ByGjS34AllFgecYw0iC6Zq7qlm8uclZFI0SqQnqBo&r=xVM0Oj0byXJqPMlhvbopPQeNDkgpFF7SJ9Qqo_aizig&m=lKsEU9Bd6rtqTfZCkUGZS3DXC1qAc6ZkHRqIuLdnYoY&s=BwM-FT6a-8hxo3GQzUp2JgtdFeXRr35XI0VKurGCWWI&e=>
*From: *Jonah Cool <jcool(a)chanzuckerberg.com>
*Date: *Saturday, 22 May 2021 at 03:04
*To: *Aaron James Wilk <awilk(a)stanford.edu>
*Cc: *Vladimir Kiselev <vk6(a)sanger.ac.uk>uk>, "cv19caingest(a)sanger.ac.uk"
<
cv19caingest(a)sanger.ac.uk>gt;, Martin Prete <mp33(a)sanger.ac.uk>uk>, Maximilian
Lombardo <mlombardo(a)chanzuckerberg.com>om>, Kirtana Veeraraghavan <
kveeraraghavan(a)chanzuckerberg.com>
*Subject: *Re: [Cv19caingest] Blish lab: New single-cell dataset for
COVID-19 cell atlas [EXT]
Hi Aaron!
Great to hear from you as always! We would be happy to host the data on
cellxgene and can work with Vlad and his team to then have the link
reflected on the covid19cellatlas site. We can take a look at the seurat
object that you shared and then circle back with some potential
questions/amendments that would be helpful or required for us to host the
data.
Have a great weekend and thanks for reaching out.
All good things,
Jonah
On Fri, May 21, 2021 at 11:33 AM Aaron James Wilk <awilk(a)stanford.edu>
wrote:
Hi Vlad,
Apologies for the extremely delayed reply. We’ve been in the midst of
revising the manuscript and had elected to wait to share the data until we
were more confident that the object we’d share would be the final one! Here
is a link to a Seurat object for the dataset:
https://urldefense.proofpoint.com/v2/url?u=https-3A__drive.google.com_file_…
[
drive.google.com]
<https://urldefense.proofpoint.com/v2/url?u=https-3A__drive.google.com_file_d_1cG60rcbff-2Dh-5F3ZoAM2GkJTttIQR4RWb9_view-3Fusp-3Dsharing&d=DwMFaQ&c=D7ByGjS34AllFgecYw0iC6Zq7qlm8uclZFI0SqQnqBo&r=9KyXPtoyUP8edjdvA3wxPg&m=K5ZPsdQyE0rEJ3G0OQbGKwCp5td6zRJhHXhWF3KrFqM&s=-fxLTy7hu5EHV8QxohQfoewblWTzSxF7SAYZXHSyuug&e=>
Please let me know if you have any issues downloading it. The manuscript is
currently in press at JEM (doi: 10.1084/jem.20210582) but not online yet.
Additionally, it would be wonderful for this dataset to be hosted on the
cellxgene platform as well. I can’t recall if that is a completely
independent process so I’ve also cc’d Jonah to remind me how to start
moving with that.
Thanks so much for your help and don’t hesitate to reach out with any
questions or concerns!
Best,
Aaron Wilk
*From: *Vladimir Kiselev <vk6(a)sanger.ac.uk>
*Date: *Thursday, January 7, 2021 at 1:57 AM
*To: *Aaron James Wilk <awilk(a)stanford.edu>du>, "cv19caingest(a)sanger.ac.uk"
<
cv19caingest(a)sanger.ac.uk>
*Cc: *Martin Prete <mp33(a)sanger.ac.uk>
*Subject: *Re: [Cv19caingest] Blish lab: New single-cell dataset for
COVID-19 cell atlas [EXT]
Hi Aaron,
Yes, sure, we can add your reference and datasets to the website. Could
you please share the datasets with us?
Many thanks,
Cheers,
Vlad
--
*Vladimir Kiselev*
CellGen Informatics
<http://www.sanger.ac.uk/science/groups/cellular-genetics-informatics>
Team Leader
Wellcome Sanger Institute
Wellcome Genome Campus
Hinxton, Cambridge, CB10 1SA
Phone: +44 (0) 1223 496850
Message: Mattermost <https://mattermost.sanger.ac.uk/cellgeninf> or Slack
[
cellgeni.slack.com]
<https://urldefense.proofpoint.com/v2/url?u=https-3A__cellgeni.slack.com_&d=DwMFaQ&c=D7ByGjS34AllFgecYw0iC6Zq7qlm8uclZFI0SqQnqBo&r=9KyXPtoyUP8edjdvA3wxPg&m=K5ZPsdQyE0rEJ3G0OQbGKwCp5td6zRJhHXhWF3KrFqM&s=WNX25Sk3kgZLGw4c8pv_tBzX8qr4UPjD2NpFGo2uiJk&e=>
Web:
https://urldefense.proofpoint.com/v2/url?u=https-3A__wikiselev.github.io&am…
[wikiselev.github.io]
<https://urldefense.proofpoint.com/v2/url?u=https-3A__wikiselev.github.io&d=DwMFaQ&c=D7ByGjS34AllFgecYw0iC6Zq7qlm8uclZFI0SqQnqBo&r=9KyXPtoyUP8edjdvA3wxPg&m=K5ZPsdQyE0rEJ3G0OQbGKwCp5td6zRJhHXhWF3KrFqM&s=lSU7imFibmiTDZzgVcBfUdpemtmzO3AgpFFYzlxuHd4&e=>
*From: *Aaron James Wilk <awilk(a)stanford.edu>
*Date: *Wednesday, 6 January 2021 at 17:17
*To: *"cv19caingest(a)sanger.ac.uk" <cv19caingest(a)sanger.ac.uk>
*Subject: *[Cv19caingest] Blish lab: New single-cell dataset for COVID-19
cell atlas [EXT]
Hi there,
My name is Aaron Wilk from the Blish lab at Stanford University. Your cell
atlas is currently hosting our early COVID-19 scRNA-seq immune profiling
work, and I am excited to tell you that we have now posted a preprint
expanding that initial work to many more patients across a wider range of
disease severity:
https://urldefense.proofpoint.com/v2/url?u=https-3A__www.biorxiv.org_conten…
[
biorxiv.org]
<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.biorxiv.org_content_10.1101_2020.12.18.423363v1&d=DwMFaQ&c=D7ByGjS34AllFgecYw0iC6Zq7qlm8uclZFI0SqQnqBo&r=9KyXPtoyUP8edjdvA3wxPg&m=K5ZPsdQyE0rEJ3G0OQbGKwCp5td6zRJhHXhWF3KrFqM&s=foYlspKfroCmRjkVWv4XHJ05sHptoF2PuUUJJAzKVgM&e=>
Would you be able to host Seurat .rds and .h5ad for this dataset as well?
Please let me know if you have any questions.
All the best,
Aaron Wilk
-- The Wellcome Sanger Institute is operated by Genome Research Limited, a
charity registered in England with number 1021457 and a company registered
in England with number 2742969, whose registered office is 215 Euston
Road, London, NW1 2BE
<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.google.com_maps_search_215-2BEuston-2BRoad-2C-2BLondon-2C-2BNW1-2B2BE-3Fentry-3Dgmail-26source-3Dg&d=DwIFaQ&c=D7ByGjS34AllFgecYw0iC6Zq7qlm8uclZFI0SqQnqBo&r=YCVDjNHxAgv8-gpjr0Xb48Y9Vk9U8oXzH6wskU6pf14&m=HCbLS-MOlRcexmQt0tb2xpS9vgwni40C0gwAXjkn3DM&s=KBT-e3Gd3eKvN7sKwr3dnq_JlK3459lBuaFLvIXxwbM&e=
>.
-- The Wellcome Sanger Institute is operated by Genome Research Limited, a
charity registered in England with number 1021457 and a company registered
in England with number 2742969, whose registered office is 215 Euston
Road, London, NW1 2BE
<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.google.com_maps_search_215-2BEuston-2BRoad-2C-2BLondon-2C-2BNW1-250D-250A-2B2BE-3Fentry-3Dgmail-26source-3Dg&d=DwIFaQ&c=D7ByGjS34AllFgecYw0iC6Zq7qlm8uclZFI0SqQnqBo&r=YCVDjNHxAgv8-gpjr0Xb48Y9Vk9U8oXzH6wskU6pf14&m=HCbLS-MOlRcexmQt0tb2xpS9vgwni40C0gwAXjkn3DM&s=2gR-6yI7ZNNyS8J7OWqny53pt4781OUM6x7awWBmsIA&e=
>.
-- The Wellcome Sanger Institute is operated by Genome Research Limited, a
charity registered in England with number 1021457 and a company registered
in England with number 2742969, whose registered office is 215 Euston
Road, London, NW1 2BE
<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.google.com_maps_search_215-2BEuston-2BRoad-2C-2BLondon-2C-2BNW1-2B2BE-3Fentry-3Dgmail-26source-3Dg&d=DwIFaQ&c=D7ByGjS34AllFgecYw0iC6Zq7qlm8uclZFI0SqQnqBo&r=YCVDjNHxAgv8-gpjr0Xb48Y9Vk9U8oXzH6wskU6pf14&m=HCbLS-MOlRcexmQt0tb2xpS9vgwni40C0gwAXjkn3DM&s=KBT-e3Gd3eKvN7sKwr3dnq_JlK3459lBuaFLvIXxwbM&e=
>.