Hi COVID-19 Atlas Team,
We have previously contributed our data to this resource (Nasal epithelia
from Shalek Group and Ordovas-Montanes lab). Our manuscript is now accepted
in principle and we would like to update the reference with the full
dataset. Could you help us with this update?
Thanks for your help!
Best,
Carly
--
Carly G. K. Ziegler
Harvard/MIT MD-PhD Program
Carly_Ziegler(a)hms.harvard.edu
carly.ziegler(a)gmail.com
404-375-8912
she/her
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Hi there,
My name is Aaron Wilk from the Blish lab at Stanford University. Your cell atlas is currently hosting our early COVID-19 scRNA-seq immune profiling work, and I am excited to tell you that we have now posted a preprint expanding that initial work to many more patients across a wider range of disease severity: https://urldefense.proofpoint.com/v2/url?u=https-3A__www.biorxiv.org_conten…
Would you be able to host Seurat .rds and .h5ad for this dataset as well?
Please let me know if you have any questions.
All the best,
Aaron Wilk
Dear COVID-19 Cell Atlas maintainer,
I downloaded haniffa21.processed.h5ad from you website twice as follows
$ wget https://covid19.cog.sanger.ac.uk/submissions/release1/haniffa21.processed.h… )
And both times
$ md5sum haniffa21.processed.h5ad
gave me
add2501947c585ea6b6ef7429e4bd9f2 haniffa21.processed.h5ad
while you provide md5 checksum
42ad74a77fc289b77cfedb7b287f7e23
Since all other files of COVID-19 PBMC Ncl-Cambridge-UCL release 1.0 matched your md5 checksums perfectly, I suspect you provide wrong checksum for haniffa21.processed.h5ad
Best Regards,
Daniil Sarkisyan, Bioinformatician
Department of Immunology, genetics and pathology, BMC B11:406b
mobile: +46 70 885 9883, email: daniil.sarkisyan(a)igp.uu.se
När du har kontakt med oss på Uppsala universitet med e-post så innebär det att vi behandlar dina personuppgifter. För att läsa mer om hur vi gör det kan du läsa här: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.uu.se_om-2Duu_datas…
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Hi John,
No problem at all and I too am excited to see the data on the covid19atlas,
which is an awesome community resource. I'm cc'ing that team here to
hopefully help with the modifications that you've pointed out.
All good things,
Jonah
On Tue, Apr 6, 2021 at 11:55 AM Tsang, John (NIH/NIAID) [E] <
john.tsang(a)nih.gov> wrote:
> Hi Jonah,
>
> Thanks so much! It has been such a pleasure working with you and Pablo on
> this and we are very grateful for your support. Excited that our dataset is
> now on the human covid19 atlas site!
>
> I noticed a couple of minor items that would benefit from small textual
> changes if possible:
>
> 1) title of the dataset -> "Blood PBMCs from COVID-19 patients over time"
> would be a better title as most of our patients were not fatal.
>
> 2) lab name - there are quite a few labs in the Laboratory of Immune
> Systems Biology (as it's a department). Easiest to put "Tsang Lab" like the
> others would be most consistent.
>
> Thanks again!
>
> John.
>
>
>
> ------------------------------
> *From:* Jonah Cool <jcool(a)chanzuckerberg.com>
> *Sent:* Tuesday, April 6, 2021 2:40 PM
> *To:* Tsang, John (NIH/NIAID) [E] <john.tsang(a)nih.gov>; Lau, William
> (NIH/NIAID) [E] <lauwill(a)mail.nih.gov>
> *Subject:* your data on covid19cellatlas
>
> John & William,
>
> Hope all is well and you've been getting lots of positive feedback on the
> paper. I wanted to quickly confirm that your data is now available (think
> it has been for a few days, actually:)) on the covid19cellatlas site
> <https://www.covid19cellatlas.org/index.patient.html> under the "patient"
> tab.
>
> Thanks again for working with us on this and looking forward to connecting
> again soon.
>
> All good things,
>
> Jonah
>
Greetings,
Thank you for making the data available for download. I have downloaded some data (https://covid19.cog.sanger.ac.uk/submissions/release1/20200804_NasalSwab_Br…), however I have been unable to open it
I get errors like this:
Warning: Unknown file type: h5ad
Warning: 'assay' not set, setting to 'RNA'
Creating h5Seurat file for version 3.1.5.9900
Adding X as data
Adding X as counts
Error: Cannot find feature names in this H5AD file
Execution halted
Or this:
error #001: c:/msys64/home/Jeroen/hdf5-1.8.16/src/H5Dio.c in H5D__read(): line 551: can't read data
class: HDF5
major: Dataset
minor: Read failed
error #002: c:/msys64/home/Jeroen/hdf5-1.8.16/src/H5Dchunk.c in H5D__chunk_read(): line 1875: unable to read raw data chunk
class: HDF5
major: Low-level I/O
minor: Read failed
error #003: c:/msys64/home/Jeroen/hdf5-1.8.16/src/H5Dchunk.c in H5D__chunk_lock(): line 2905: data pipeline read failed
class: HDF5
major: Data filters
minor: Filter operation failed
error #004: c:/msys64/home/Jeroen/hdf5-1.8.16/src/H5Z.c in H5Z_pipeline(): line 1347: required filter 'lzf' is not registered
class: HDF5
major: Data filters
minor: Read failed
There are many message boards that have listed possible solutions, but I have not been able to get any of them to work. Could you provide some guidance as to what programs are used to open/manipulate these files please?
Sincerely,
Erin
Hi,
I previously contributed data to the COVID-19 Cell Atlas. I now have an updated/finalized Fetal Cerebellum dataset that I would like to replace for the dataset that is currently hosted: https://www.covid19cellatlas.org/index.healthy.html
What is the best way to transfer the updated .rds?
Thanks!
Kimberly A Aldinger, PhD (she/her)
Center for Integrative Brain Research (CIBR)
Seattle Children's Research Institute
Member, Brotman Baty Institute for Precision Medicine
206-884-1226 OFFICE
206-884-1210 FAX
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